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tianscript m mlv  (tiangen biotech co)


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    Structured Review

    tiangen biotech co tianscript m mlv
    Tianscript M Mlv, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tianscript+m+mlv/TIANScript+M-MLV/pmc11997863-140-9-11
    Average 96 stars, based on 51 article reviews
    tianscript m mlv - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Long non‐coding RNA HCG11 modulates glioma progression through cooperating with miR‐496/CPEB3 axis
    Article Snippet: Reverse transcription was finished by using PrimeScriptTM RT Master Mix (TaKaRa, Dalian, China). .. To detect the expression level of miRNA, the reverse transcription of miRNAs was carried out with TIANScript M‐MLV (Tiangen, Beijing, China). qRT‐PCR was conducted on a LightCycler 480 instrument (Roche, Basel, Switzerland) using SYBR Premix Ex Taq II (TaKaRa). ..

    Article Title: Serum miR-551b-3p is a potential diagnostic biomarker for gastric cancer
    Article Snippet: Total RNA was extracted from 300 μl serum using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Then, the RNA was reverse transcribed using TIANScript M-MLV (Tiangen Biotech Beijing Co., Ltd., Beijing, China). hsa-miRNA-16 (miR-16) was used as an internal reference in the present study. qPCR to detect the expression levels of miR-551b-3p was performed using a LightCycler PCR System (Roche, Mannheim, Germany) with a 10 μl quantitative reverse transcription polymerase chain reaction (RT-qPCR) reaction mixture, comprising forward primer (0.5 μl), reverse primer (0.5 μl), cDNA (2 μl), SYBR Premix Ex Taq II (Tli RNase H Plus, 2×) (5 μl), and RNase/DNase-free water (2 μl). ..

    Reverse Transcription:

    Article Title: Long non‐coding RNA HCG11 modulates glioma progression through cooperating with miR‐496/CPEB3 axis
    Article Snippet: Reverse transcription was finished by using PrimeScriptTM RT Master Mix (TaKaRa, Dalian, China). .. To detect the expression level of miRNA, the reverse transcription of miRNAs was carried out with TIANScript M‐MLV (Tiangen, Beijing, China). qRT‐PCR was conducted on a LightCycler 480 instrument (Roche, Basel, Switzerland) using SYBR Premix Ex Taq II (TaKaRa). ..

    Article Title: Nicotinamide increases the sensitivity of chronic myeloid leukemia cells to doxorubicin via the inhibition of SIRT1.
    Article Snippet: Funding information Hubei Provincial Natural Science Fund for Creative Research Groups, Grant/ Award Number: 2018CFA018; National Natural Science Foundation of China, Grant/Award Numbers: 81501352, 81770180, 81500151, 81270607, 81400121 Abstract The NAD‐dependent deacetylase Sirtuin 1 (SIRT1) plays a vital role in leukemogenesis.. Nicotinamide (NAM) is the principal NAD precursor and a noncompetitive inhibitor of SIRT1.. In our study, we showed that NAM enhanced the sensitivity of chronic myeloid leukemia (CML) to doxorubicin (DOX) via SIRT1.

    Article Title: MicroRNA-410-5p exacerbates high-fat diet-induced cardiac remodeling in mice in an endocrine fashion.
    Article Snippet: For measurement of mRNA levels, total RNA from tissue samples was isolated using RNAprep Pure Tissue kit (Tiangen, Beijing, China) according to the manufacturer’s instructions. .. Random-primed cDNAs were generated by reverse transcription of total RNA with TIANScript M-MLV (Tiangen). .. A real-time PCR analysis was performed with the ABI Prism 7000 Sequence Detection system (Applied Biosystems) using SYBR® Premix-Ex TagTM (Takara, Dalian, Chnia).

    Article Title: Serum miR-551b-3p is a potential diagnostic biomarker for gastric cancer
    Article Snippet: Total RNA was extracted from 300 μl serum using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Then, the RNA was reverse transcribed using TIANScript M-MLV (Tiangen Biotech Beijing Co., Ltd., Beijing, China). hsa-miRNA-16 (miR-16) was used as an internal reference in the present study. qPCR to detect the expression levels of miR-551b-3p was performed using a LightCycler PCR System (Roche, Mannheim, Germany) with a 10 μl quantitative reverse transcription polymerase chain reaction (RT-qPCR) reaction mixture, comprising forward primer (0.5 μl), reverse primer (0.5 μl), cDNA (2 μl), SYBR Premix Ex Taq II (Tli RNase H Plus, 2×) (5 μl), and RNase/DNase-free water (2 μl). ..

    Random Primed:

    Article Title: MicroRNA-410-5p exacerbates high-fat diet-induced cardiac remodeling in mice in an endocrine fashion.
    Article Snippet: For measurement of mRNA levels, total RNA from tissue samples was isolated using RNAprep Pure Tissue kit (Tiangen, Beijing, China) according to the manufacturer’s instructions. .. Random-primed cDNAs were generated by reverse transcription of total RNA with TIANScript M-MLV (Tiangen). .. A real-time PCR analysis was performed with the ABI Prism 7000 Sequence Detection system (Applied Biosystems) using SYBR® Premix-Ex TagTM (Takara, Dalian, Chnia).

    Generated:

    Article Title: MicroRNA-410-5p exacerbates high-fat diet-induced cardiac remodeling in mice in an endocrine fashion.
    Article Snippet: For measurement of mRNA levels, total RNA from tissue samples was isolated using RNAprep Pure Tissue kit (Tiangen, Beijing, China) according to the manufacturer’s instructions. .. Random-primed cDNAs were generated by reverse transcription of total RNA with TIANScript M-MLV (Tiangen). .. A real-time PCR analysis was performed with the ABI Prism 7000 Sequence Detection system (Applied Biosystems) using SYBR® Premix-Ex TagTM (Takara, Dalian, Chnia).

    Real-time Polymerase Chain Reaction:

    Article Title: Serum miR-551b-3p is a potential diagnostic biomarker for gastric cancer
    Article Snippet: Total RNA was extracted from 300 μl serum using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Then, the RNA was reverse transcribed using TIANScript M-MLV (Tiangen Biotech Beijing Co., Ltd., Beijing, China). hsa-miRNA-16 (miR-16) was used as an internal reference in the present study. qPCR to detect the expression levels of miR-551b-3p was performed using a LightCycler PCR System (Roche, Mannheim, Germany) with a 10 μl quantitative reverse transcription polymerase chain reaction (RT-qPCR) reaction mixture, comprising forward primer (0.5 μl), reverse primer (0.5 μl), cDNA (2 μl), SYBR Premix Ex Taq II (Tli RNase H Plus, 2×) (5 μl), and RNase/DNase-free water (2 μl). ..

    Polymerase Chain Reaction:

    Article Title: Serum miR-551b-3p is a potential diagnostic biomarker for gastric cancer
    Article Snippet: Total RNA was extracted from 300 μl serum using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. Then, the RNA was reverse transcribed using TIANScript M-MLV (Tiangen Biotech Beijing Co., Ltd., Beijing, China). hsa-miRNA-16 (miR-16) was used as an internal reference in the present study. qPCR to detect the expression levels of miR-551b-3p was performed using a LightCycler PCR System (Roche, Mannheim, Germany) with a 10 μl quantitative reverse transcription polymerase chain reaction (RT-qPCR) reaction mixture, comprising forward primer (0.5 μl), reverse primer (0.5 μl), cDNA (2 μl), SYBR Premix Ex Taq II (Tli RNase H Plus, 2×) (5 μl), and RNase/DNase-free water (2 μl). ..

    cDNA Synthesis:

    Article Title: Green synthesis of silver nanoparticles using the BT5 tea cultivar of Bangladesh: unveiling molecular mechanisms of anti-cancer activity in mice model
    Article Snippet: Nanodrop (Thermo Fisher, Madison, USA) was used for the quality assessment and quantification of RNA. .. First-strand complementary DNA (cDNA) synthesis was performed using the TIANScript M-MLV (Tiangen R , Beijing, China) protocol. ..



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